TY - JOUR ID - 668 TI - Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma JO - Iranian Journal of Otorhinolaryngology JA - IJORL LA - en SN - 2251-7251 AU - Pardis, Soheil AU - Sardari, Yasaman AU - Ashraf, Mohammad Javad AU - Tadbir, Azadeh Andisheh AU - Ebrahimi, Hooman AU - Purshahidi, Sara AU - Khademi, Bijan AU - Fattahi, Mohammad Javad AU - Hamzavi, Marzieh AD - Department of Oral and MaxillofacialPathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Department of Oral Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Department of Otolaryngology, Khalili Hospital, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran. Y1 - 2012 PY - 2012 VL - 24 IS - 4 SP - 161 EP - 170 KW - Carcinoma KW - HER2/neu KW - salivary gland KW - Squamous cell of head and neck KW - Tissue expression DO - 10.22038/ijorl.2012.668 N2 - Introduction: HER2/neu, a member of the epidermal growth factor receptor family, has been shown to be over-expressed in some tumors. The purpose of this study was to determine the salivary levels and tissue expression of HER2/neu in patients with head and neck squamous cell carcinoma (HNSCC) and their correlation with clinicopathologic parameters.   Materials and Methods: Enzyme-linked immunosorbent assays (ELISA) were used to evaluate the salivary levels of HER2/neu and immunohistochemistry was used to measure tissue expression of HER2/neu in 28 patients with HNSCC and 25 healthy control subjects.   Results: The salivary levels of HER2/neu in patients with HNSCC were not significantly higher compared to healthy control subjects. There was no apparent correlation between salivary HER2/neu levels and clinicopathological features such as age, sex, tumor grade, tumor size and nodal status. All HNSCC specimens were positive (membranous or/and cytoplasmic) for HER2/neu, except one sample. Only one HNSCC specimen showed staining purely in the tumor-cell cytoplasm. All control specimens were also positive for both membranous and cytoplasmic HER2/neu but there was a significant difference between the level of cytoplasmic staining in the HNSCC specimens and in the control specimens (P<0.05).   Conclusion: In our study, no overexpression of HER2/neu was observed. Thus, identification of HER2/neu levels plays no role in differentiating between normal and squamous cell carcinoma tissues or detecting the carcinogenesis process. Our findings suggest that the use of HER2/neu as a salivary marker of HNSCC is not recommended, because no significant preoperative elevation and no association with clinicopathological features were found.  UR - https://ijorl.mums.ac.ir/article_668.html L1 - https://ijorl.mums.ac.ir/article_668_b706dce7bea8b8f23e9327611db6360d.pdf ER -